Related Experiment Videos
High throughput on-line solid phase extraction/tandem mass spectrometric determination of paclitaxel in human serum
A Schellen1, B Ooms, M van Gils
1Spark Holland BV, P.O. Box 388, 7800 AJ Emmen, The Netherlands.
Rapid Communications in Mass Spectrometry : RCM
|February 12, 2000
Abstract:
The feasibility of high throughput on-line solid phase extraction/tandem mass spectrometry (SPE/MS/MS) is tested for target analysis of paclitaxel in human serum. The use of a dual Prospekt system, with parallel SPE and elution directly to the mass spectrometer, resulted in a cycle time of 80 seconds for the entire, fully automated assay. The assay proved to be linear from 1 to 1000 ng/mL. Cartridges packed with small sorbent particles functioned both as SPE cartridges and as short analytical columns.