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Updated: Oct 17, 2026
Monitoring Functionality and Morphology of Vasculature Recruited by Factors Secreted by Fast-growing Tumor-generating Cells
Published on: November 23, 2014
Biological parameters for the choice of antiangiogenic therapy and efficacy monitoring
M Ziche1, C Sorriso, A Parenti
1Interuniversity Center of Molecular Medicine and Applied Biophysics, Medical School, University of Firenze, Italy. ziche@ds.unifi.it
Abstract:
Angiogenesis is a tightly controlled process which depends on the balance between stimulating and inhibiting factors. When this balance is disrupted, angiogenesis acquires a pathological meaning. The list of molecules able to induce angiogenesis is heterogeneous with respect to their chemical characteristics and biological properties. Quantitative measurement of tumor angiogenesis is necessary for the choice of therapeutic strategies and as an endpoint for antiangiogenic therapy. We are developing a quantitative RT-PCR with measures the expression of specific factors in real time. With the use of this rapid technique, measurement of the expression of the angiogenic factors and inhibitors is also possible in specimens as small as biopsies.
Insights
This study introduces a quantitative RT-PCR method for measuring tumor angiogenesis factors. This technique enables rapid assessment of angiogenic factors and inhibitors, even in small biopsy samples.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- Angiogenesis, the formation of new blood vessels, is crucial for tumor growth and metastasis.
- A disruption in the balance of pro-angiogenic and anti-angiogenic factors can lead to pathological angiogenesis.
- Accurate measurement of tumor angiogenesis is essential for selecting effective therapeutic strategies and evaluating anti-angiogenic treatments.
Purpose of the Study:
- To develop and validate a quantitative real-time PCR (RT-PCR) assay for measuring the expression of specific angiogenic factors and inhibitors.
- To establish a rapid and sensitive method for the quantitative assessment of tumor angiogenesis.
Main Methods:
- Development of a quantitative RT-PCR assay.
- Real-time measurement of gene expression for angiogenic factors and inhibitors.
- Application of the technique to small tissue specimens, including biopsies.
Main Results:
- The quantitative RT-PCR method allows for rapid and accurate measurement of angiogenic factor expression.
- The technique is sensitive enough to quantify gene expression in limited sample sizes, such as biopsies.
- This method provides a tool for assessing the angiogenic potential of tumors.
Conclusions:
- Quantitative RT-PCR offers a valuable tool for the precise measurement of tumor angiogenesis.
- This technique facilitates the assessment of angiogenic factors and inhibitors in clinical settings, aiding therapeutic decisions.
- The ability to analyze small biopsies enhances diagnostic capabilities for angiogenesis-related pathologies.
