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High-sensitivity chromatographic detector incorporating three-dimensional charge coupled device fluorimetry
1Cranfield Centre for Analytical Science, IBST, Cranfield University, Bedford, UK.
Journal of Chromatography. A
|February 12, 2000
Summary
This study introduces a novel liquid chromatography detector for analyzing fluorescent compounds. The instrument rapidly captures 3D excitation-emission-intensity data, achieving high sensitivity for polycyclic aromatic hydrocarbons.
Area of Science:
- Analytical Chemistry
- Spectroscopy
- Chromatography
Background:
- Fluorescent compounds are crucial in various scientific fields.
- Accurate detection and quantification of these compounds are essential.
- Existing methods may lack the speed or sensitivity required for certain applications.
Purpose of the Study:
- To develop and describe a new detector for liquid chromatography.
- To enable three-dimensional, excitation-emission-intensity data acquisition.
- To achieve sensitive detection of fluorescent compounds in a flow stream.
Main Methods:
- Utilized a polychromator for multi-wavelength excitation dispersed along a quartz capillary flow cell.
- Employed an emission polychromator to collect and disperse fluorescence.
- Used a charge-coupled device (CCD) pixel array to capture the two-dimensional excitation-emission image and intensity data.
Main Results:
- The detector provides three-dimensional excitation-emission-intensity data.
- Achieved rapid data collection (less than 0.05 seconds) for polycyclic aromatic hydrocarbons.
- Demonstrated nanogram per milliliter limits of detection.
Conclusions:
- The dual polychromator and CCD arrangement offers extreme sensitivity.
- This detector is suitable for rapid, sensitive analysis of fluorescent compounds in liquid chromatography.
- The instrument advances the capability for analyzing polycyclic aromatic hydrocarbons in flow streams.