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Updated: Jul 29, 2026

Mechanical Stimulation-induced Calcium Wave Propagation in Cell Monolayers: The Example of Bovine Corneal Endothelial Cells
Published on: July 16, 2013
Analysis of connexin intracellular transport and assembly
1Vollum Institute, Oregon Health Sciences University, 3181 Southwest Sam Jackson Park Road, Portland, Oregon 97201, USA.
Abstract:
Central to understanding the establishment and regulation of gap junction-mediated intercellular communication is a detailed knowledge of how these complex structures are assembled. In this article, we describe methods to modulate and/or monitor the transport of connexins from the endoplasmic reticulum to the Golgi complex and from the trans Golgi network to the cell surface. We also present techniques that we have developed to assay assembly of newly synthesized connexin monomers into connexons and gap junctional plaques.
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