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Related Experiment Videos

DNA uptake signal sequences in naturally transformable bacteria.

H O Smith1, M L Gwinn, S L Salzberg

  • 1The Institute for Genomic Research, Rockville, MD 20850, USA. SmithHO@celera.com

Research in Microbiology
|February 15, 2000
PubMed
Summary

Naturally transformable bacteria like Haemophilus influenzae Rd and Neisseria meningitidis utilize DNA uptake signal sequences (USS). These crucial USS sequences are frequently located within transcription terminators, facilitating genetic exchange.

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Area of Science:

  • Microbiology
  • Genetics
  • Molecular Biology

Background:

  • Naturally transformable bacteria possess mechanisms for DNA uptake.
  • DNA uptake signal sequences (USS) are essential for this process in some species.
  • The distribution and function of USS in bacterial genomes are not fully understood.

Purpose of the Study:

  • To investigate the prevalence and location of DNA uptake signal sequences (USS) in naturally transformable bacteria.
  • To identify specific USS sequences in Haemophilus influenzae Rd and Neisseria meningitidis.
  • To explore the association between USS and transcriptional regulatory elements.

Main Methods:

  • Bioinformatic analysis of bacterial genomes.
  • Sequence identification and counting of USS.

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  • Comparative genomics.

    • Main Results:

    • Haemophilus influenzae Rd contains 1471 copies of the 5'-AAGTGCGGT USS.
    • Neisseria meningitidis contains 1891 copies of the 5'-GCCGTCTGAA USS.
    • USS are frequently found within the stem regions of transcription terminators.

    Conclusions:

    • Naturally transformable bacteria possess a high number of specific USS.
    • The location of USS within transcription terminators suggests a regulatory role in DNA uptake.
    • Further research is needed to elucidate the precise function of USS in gene regulation and horizontal gene transfer.