Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A novel TNF-inducible message with putative growth suppressor function.

S S Nadadur1, M J Ehrke, H L Gurtoo

  • 1Department of Pharmacology and Therapeutics, Grace Cancer Drug Center, Roswell Park Cancer Institute, Buffalo, NY 14263, USA. nadadur.srikanth@epamail.epa.gov

Biochimica Et Biophysica Acta
|February 15, 2000
PubMed
Summary

Researchers identified a novel gene

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Iron transport & homeostasis mechanisms: their role in health & disease.

The Indian journal of medical research·2008
Same author

Expression, tissue distribution, and cellular localization of the antiapoptotic TIP-B1 protein.

Journal of leukocyte biology·2001
Same author

Changes in cytosolic and membrane TNF inhibitory protein-B1 (TIP-B1) levels associated with protection from TNF-induced cytotoxicity.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2001
Same author

Anticancer drugs plus cytokines: immunodulation based therapies of mouse tumors.

International journal of immunopharmacology·2001
Same author

A novel tumor necrosis factor-alpha inhibitory protein, TIP-B1.

International journal of immunopharmacology·2001
Same author

A pulmonary rat gene array for screening altered expression profiles in air pollutant-induced lung injury.

Inhalation toxicology·2000

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Genetics

Background:

  • Tumorigenesis involves complex genetic alterations.
  • Gene expression profiling is crucial for understanding cancer.
  • Tumor necrosis factor (TNF) plays a role in cellular responses.

Purpose of the Study:

  • To identify and characterize a novel cDNA sequence.
  • To investigate the expression of the novel message (mRNA) in human cells.
  • To explore the potential role of this gene in cell growth regulation.

Main Methods:

  • Nucleotide sequencing of novel cDNA.
  • Analysis of TNF-induced mRNA expression in fibroblast cells.
  • Transfection assays using sense and antisense constructs in NIH3T3 cells.

Related Experiment Videos

Main Results:

  • A novel cDNA sequence was identified.
  • The corresponding mRNA is expressed in human fibroblasts and tumor cell lines, with overexpression in colon cancer.
  • Antisense constructs targeting the 5'-region significantly increased colony formation in NIH3T3 cells.

Conclusions:

  • The novel gene may function as a growth suppressor.
  • Its altered expression in colon cancer suggests a role in tumorigenesis.
  • Further investigation into its mechanism is warranted.