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A novel TNF-inducible message with putative growth suppressor function
S S Nadadur1, M J Ehrke, H L Gurtoo
1Department of Pharmacology and Therapeutics, Grace Cancer Drug Center, Roswell Park Cancer Institute, Buffalo, NY 14263, USA. nadadur.srikanth@epamail.epa.gov
Abstract:
We report the nucleotide sequence of a novel cDNA and TNF-induced expression of the corresponding message (mRNA) in human fibroblast cells. This message is also expressed in certain human tumor cell lines and is over-expressed in a colon cancer cell line (HT-29). NIH3T3 cells transfected with the antisense construct of the 5'-region of this novel cDNA formed 20-fold more colonies in culture compared to cells transfected with a sense construct of the same region or the sense and the antisense constructs of the central region of this cDNA. This observation suggests a possible growth suppressor function for the gene represented by this cDNA.
Insights
Researchers identified a novel gene
Area of Science:
- Molecular Biology
- Cancer Research
- Genetics
Background:
- Tumorigenesis involves complex genetic alterations.
- Gene expression profiling is crucial for understanding cancer.
- Tumor necrosis factor (TNF) plays a role in cellular responses.
Purpose of the Study:
- To identify and characterize a novel cDNA sequence.
- To investigate the expression of the novel message (mRNA) in human cells.
- To explore the potential role of this gene in cell growth regulation.
Main Methods:
- Nucleotide sequencing of novel cDNA.
- Analysis of TNF-induced mRNA expression in fibroblast cells.
- Transfection assays using sense and antisense constructs in NIH3T3 cells.
Main Results:
- A novel cDNA sequence was identified.
- The corresponding mRNA is expressed in human fibroblasts and tumor cell lines, with overexpression in colon cancer.
- Antisense constructs targeting the 5'-region significantly increased colony formation in NIH3T3 cells.
Conclusions:
- The novel gene may function as a growth suppressor.
- Its altered expression in colon cancer suggests a role in tumorigenesis.
- Further investigation into its mechanism is warranted.