A simple screening assay for certain fibrinolysis parameters (FIPA)

Area of Science:

• Biochemistry
• Hematology
• Clinical Diagnostics

Background:

• Hemostasis involves coagulation and fibrinolysis, the generation and degradation of thrombi.
• Existing global assays for coagulation are established, but rapid, economical tests for plasmatic fibrinolysis parameters are lacking.
• Key plasmatic fibrinolysis parameters include plasminogen activator inhibitor-1, alpha2-antiplasmin, plasminogen, and aprotinin.

Purpose of the Study:

• To develop a fast, functional, and economical global assay for routine measurement of plasmatic fibrinolysis parameters.
• To establish optimal conditions for the assay to accurately detect plasmatic fibrinolytic activity.

Main Methods:

• A novel functional global assay was developed using citrated plasma incubated with urokinase, tranexamic acid, and other reagents.
• Plasmin generation and detection phases were optimized using specific concentrations and incubation times at 37°C.
• Assay results were calibrated against pooled normal plasma, and intra-assay/inter-assay variations were assessed.

Main Results:

• The assay demonstrated high reproducibility with coefficients of variation less than 5%.
• The functional fibrinolysis assay has a detection limit of 5% of normal plasmatic fibrinolysis parameters activity.
• A significant negative correlation (r = -0.684) was observed between the assay results and plasminogen activator inhibitor-1 activity in patient samples.

Conclusions:

• The developed assay is a simple, rapid, and economic functional test for multiple clinically relevant plasmatic fibrinolysis parameters.
• This assay addresses the need for a routine test to assess fibrinolytic activity.
• The assay shows potential for clinical application in evaluating hemostasis and thrombosis disorders.