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Related Experiment Videos

Inflammatory cytokines alter human gallbladder epithelial cell absorption/secretion.

R V Rege1

  • 1Division of Gastrointestinal and Endocrine Surgery, University of Texas Southwestern Medical Center, Dallas, TX 75235-9156, USA. rrege@mednet.swnet.edu

Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract
|February 16, 2000
PubMed
Summary

Inflammatory cytokines like interleukin-1 (IL-1) and tumor necrosis factor (TNF-alpha) directly impair gallbladder epithelial cell function. This disruption of absorptive processes may contribute to gallstone formation.

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Area of Science:

  • Gastroenterology
  • Cell Biology
  • Pathophysiology

Background:

  • Gallbladder inflammation is an early indicator of gallstone development in animal models.
  • Inflammation involves increased myeloperoxidase and interleukin (IL)-1 activity in the gallbladder wall.

Purpose of the Study:

  • To investigate if inflammatory cytokines directly impact the absorptive function of gallbladder epithelial cells.
  • To understand the role of altered absorptive function in gallstone pathogenesis.

Main Methods:

  • Cultured human gallbladder epithelial cells were exposed to various agents, including interleukin-1 alpha (IL-1alpha), tumor necrosis factor alpha (TNF-alpha), lipopolysaccharide, and prostaglandin E2.
  • Unidirectional sodium and chloride fluxes were measured to calculate net ion transport.

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  • The effect of IL-1alpha was assessed with and without its specific receptor antagonist, IL-1ra.

    • Main Results:

    • Lipopolysaccharide, prostaglandin E2, IL-1alpha, and TNF-alpha significantly reduced net sodium and chloride absorption across gallbladder epithelial cells.
    • These agents also increased the movement of sodium and other ions from the serosal to the mucosal side.
    • The inhibitory effects of IL-1alpha on ion transport were fully blocked by IL-1ra.

    Conclusions:

    • Proinflammatory cytokines IL-1alpha and TNF-alpha directly impair gallbladder epithelial cell absorptive function, similar to lipopolysaccharide and prostaglandin E2.
    • Altered gallbladder absorptive function due to inflammation may play a critical role in the development of gallstones.