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Updated: Aug 17, 2026

Determination of the Gas-phase Acidities of Oligopeptides
Published on: June 24, 2013
Determination of aliphatic anhydrides and acids by reversed-phase liquid chromatography
G W Tindall1, R L Perry, A T Spaugh
1Eastman Chemical Company, Kingsport, TN 37662, USA. tindall@eastman.com
Abstract:
Few chromatography methods have been reported for the determination of anhydrides in mixtures or as mixed anhydrides. The potential reactivity of anhydrides with water and other common eluent components complicates possible schemes for separation and analysis. By optimizing variables that affect hydrolysis, including the stationary phase, conditions can be found to successfully analyze anhydrides as reactive as acetic anhydride. The corresponding acids can be determined at the same time. The effect of the stationary phase on anhydride hydrolysis rates may prove to be a sensitive means of probing stationary phase chemistry.
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