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Updated: Jul 31, 2026

Design and Operation of a Continuous 13C and 15N Labeling Chamber for Uniform or Differential, Metabolic and Structural, Plant Isotope Labeling
Published on: January 16, 2014
Abstract:
A new heated water-bath humidifier operates on a new basic principle which overcomes the practical disadvantages of existing systems. A heated hose is used to control a temperature drop along the whole length of the delivery line instead of raising the temperature of the gases in the delivery line as in previous systems. Therefore the tank does not have to fully saturate the gases and a simple, totally cleanable design is possible. A temperature sensor at the delivery point controls the hose heater, constituting a rapid response, low thermal inertia system and delivery temperature is displayed on the unit. Compensation for varying gas flows and ambient temperature changes is inherent and the unit is suitable for neonatal/paediatric and adult use without special adjustment. The tank has a separate reservoir chamber which feeds an evaporation chamber via a cleanable float valve, conferring many advantages including clearly visible water levels, easy filling without breaking the circuit and constant compression volume. Full fail-safe alarm systems are incorporated.
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