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Related Experiment Videos

The alpha-mannosidases: phylogeny and adaptive diversification.

D S Gonzalez1, I K Jordan

  • 1Department of Medical Microbiology, University of Georgia, USA.

Molecular Biology and Evolution
|March 11, 2000
PubMed
Summary

Alpha-mannosidase enzymes, crucial for glycoprotein processing, show evolutionary diversification. Gene duplication events in this superfamily may be driven by positive Darwinian selection, shaping functional variants.

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Sequence and structural aspects of functional diversification in class I alpha-mannosidase evolution.

Bioinformatics (Oxford, England)·2001

Area of Science:

  • Biochemistry
  • Evolutionary Biology
  • Genomics

Background:

  • Alpha-mannosidase enzymes are glycoside hydrolases vital for glycoprotein-linked oligosaccharide maturation and degradation.
  • These enzymes belong to an ancient and widespread gene superfamily with diverse enzymatic activities.

Purpose of the Study:

  • To characterize the evolutionary relationships and dynamics of the alpha-mannosidase superfamily.
  • To investigate the functional diversification of alpha-mannosidase paralogs and orthologs.
  • To assess the role of natural selection in the evolution of alpha-mannosidase functional diversification.

Main Methods:

  • Comparative sequence analysis using lineage-specific BLAST searches.
  • Motif-based alignment and phylogenetic analysis of the alpha-mannosidase superfamily.

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  • Analysis of nucleotide polymorphism and ratios of nonsynonymous and synonymous variation.

    • Main Results:

    • Identification of the first archaean and eubacterial alpha-mannosidase sequences, alongside eukaryotic ones.
    • Discovery of three well-supported monophyletic clades representing distinct alpha-mannosidase families.
    • Evidence of functional diversification in paralogs and conservation in orthologs, with increased nonsynonymous variation post-duplication.

    Conclusions:

    • The study reveals the evolutionary history and diversification patterns within the alpha-mannosidase superfamily.
    • Functional diversification of alpha-mannosidase paralogs appears to be influenced by positive Darwinian selection following gene duplication.