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Cloning and characterization of a novel adaptor protein, CIN85, that interacts with c-Cbl

H Take1, S Watanabe, K Takeda

  • 1Hematology Branch, National Institutes of Health, Bethesda, Maryland, 20892, USA.

Biochemical and Biophysical Research Communications
|February 19, 2000
PubMed

Insights

Researchers discovered CIN85, a novel protein that interacts with c-Cbl. This interaction is crucial for epidermal growth factor (EGF) receptor signaling pathways.

Area of Science:

  • Molecular and Cellular Biology
  • Signal Transduction
  • Oncogene Research

Background:

  • The c-Cbl proto-oncogene product is a key substrate for protein tyrosine kinases.
  • c-Cbl undergoes rapid tyrosine phosphorylation upon stimulation of various cell-surface receptors, indicating its role in signaling pathways.

Purpose of the Study:

  • To identify novel proteins interacting with c-Cbl.
  • To investigate the role of a newly identified c-Cbl-interacting protein, CIN85, in cellular signaling.

Main Methods:

  • Identification and characterization of CIN85, an 85 kDa protein.
  • Analysis of CIN85 mRNA expression in human tissues and cancer cell lines.
  • Investigation of CIN85-c-Cbl interaction using biochemical assays, focusing on the role of CIN85's SH3 domains.
  • Stimulation of 293 cells with epidermal growth factor (EGF) to study dynamic changes in CIN85-c-Cbl association and c-Cbl phosphorylation.

Main Results:

  • A novel c-Cbl-interacting protein, CIN85, was identified, sharing similarities with adaptor proteins CMS and CD2AP.
  • CIN85 mRNA is ubiquitously expressed in normal human tissues and cancer cell lines.
  • CIN85 was found to be constitutively associated with c-Cbl, with the second SH3 domain of CIN85 being critical for this interaction.
  • The association between CIN85 and c-Cbl was transiently enhanced upon EGF stimulation, correlating with c-Cbl tyrosine phosphorylation levels.

Conclusions:

  • CIN85 is a novel adaptor protein that interacts with c-Cbl.
  • The interaction between CIN85 and c-Cbl is regulated by EGF stimulation and c-Cbl phosphorylation.
  • CIN85 likely plays a specific role in the epidermal growth factor receptor (EGFR) signaling cascade through its interaction with c-Cbl.

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