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E-Selectin expression in a murine model of chronic colitis
S Kawachi1, Z Morise, E Conner
1Department of Molecular and Cellular Physiology, LSU Health Sciences Center, Shreveport, Louisiana 71130, USA.
Biochemical and Biophysical Research Communications
|February 19, 2000
Summary
Chronic colitis in mice is induced by CD45RB(high) T-cells, leading to increased E-selectin expression in the colon and other organs. This study quantifies E-selectin in a T-cell model of colitis.
Area of Science:
- Immunology
- Gastroenterology
- Cell Biology
Background:
- Chronic colitis is a significant health concern.
- Understanding the molecular mechanisms of colitis is crucial for developing effective treatments.
- E-selectin is an adhesion molecule implicated in inflammatory processes.
Purpose of the Study:
- To quantify E-selectin surface expression in the colon and other tissues in a mouse model of chronic colitis.
- To investigate the role of CD4(+) T-cell subsets in the induction of colitis and E-selectin expression.
Main Methods:
- Utilized a dual radiolabel monoclonal antibody technique to quantify E-selectin expression.
- Employed a CD4(+) T-cell transfer model in severe combined immunodeficient (SCID) mice.
- Administered CD4(+)CD45RB(low) or CD45RB(high) T-cells to SCID mice and monitored for colitis development.
Main Results:
- Mice reconstituted with CD45RB(high) T-cells developed chronic colitis, characterized by weight loss, diarrhea, and histopathological changes.
- Enhanced E-selectin expression was observed in the colon of colitic mice compared to controls.
- Increased E-selectin expression was also detected in the cecum, small intestine, mesentery, and liver of colitic mice.
Conclusions:
- Reconstitution of SCID mice with CD45RB(high) T-cells reliably induces chronic colitis.
- Chronic colitis in this model is associated with enhanced E-selectin expression.
- E-selectin upregulation occurs in multiple tissues, extending beyond the primary site of inflammation.