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Related Experiment Videos

Cathepsins and compartmentalization in antigen presentation.

R J Riese1, H A Chapman

  • 1Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.

Current Opinion in Immunology
|February 19, 2000
PubMed
Summary
This summary is machine-generated.

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The invariant chain

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • MHC class II molecules are crucial for adaptive immunity.
  • Their cell surface expression depends on the invariant chain chaperone.
  • Invariant chain processing is key to MHC class II maturation.

Purpose of the Study:

  • To investigate the role of endosomal proteases in MHC class II trafficking.
  • To explore how invariant chain proteolysis impacts MHC class II cell surface expression.
  • To understand the influence of cystatin C on dendritic cell maturation and MHC class II presentation.

Main Methods:

  • Inhibition of endosomal proteases.
  • Analysis of MHC class II complex trafficking.
  • Assessment of cell surface expression of MHC class II molecules.

Related Experiment Videos

  • Evaluation of cystatin C expression in dendritic cells.

    • Main Results:

    • Inhibiting endosomal proteases causes MHC class II complexes to be shunted to lysosomes.
    • Cystatin C regulates endosomal protease activity.
    • Cystatin C expression directs MHC class II cell surface expression during dendritic cell maturation.

    Conclusions:

    • The fate of the invariant chain is critical for MHC class II trafficking and expression.
    • Endosomal protease activity, modulated by cystatin C, is a key regulatory point in MHC class II maturation.
    • Taut interactions between MHC class II-invariant chain complexes and endosomal proteases govern MHC class II presentation.