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Filamin isogene expression during mouse myogenesis.

W Chiang1, M L Greaser, G E Lyons

  • 1Muscle Biology Laboratory, University of Wisconsin-Madison, Madison, WI 53706, USA.

Developmental Dynamics : an Official Publication of the American Association of Anatomists
|February 19, 2000
PubMed
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This study reveals that muscle-specific filamin gene expression begins before myotube formation in mouse embryos. These findings offer insights into early skeletal muscle development and filamin gene regulation.

Area of Science:

  • Developmental biology
  • Molecular genetics
  • Cell biology

Background:

  • Filamin proteins are crucial cytoskeletal components involved in various cellular processes.
  • Understanding the developmental expression patterns of filamin isoforms is essential for comprehending muscle development.

Purpose of the Study:

  • To investigate the temporal and spatial expression patterns of muscle-specific and nonmuscle-specific filamin genes during mouse embryonic development.
  • To determine the onset of muscle-specific filamin gene expression relative to myotube formation.

Main Methods:

  • Utilized isoform-specific, (35)S-labeled antisense complementary ribonucleic acids (cRNAs) for in situ hybridization.
  • Employed Northern blot analysis to examine filamin transcript sizes and expression levels.

Related Experiment Videos

  • Analyzed filamin gene expression in mouse embryos at various developmental stages.

    • Main Results:

    • Nonmuscle-specific filamin transcripts (9.5 kb) were ubiquitously expressed in nonmuscle tissues, with high levels in epithelial and smooth muscle cells.
    • Muscle-specific filamin transcripts (10 kb) were primarily detected in cardiac and skeletal muscle tissues.
    • Muscle-specific filamin mRNA expression was observed in the heart by 8.0 days post-coitum and in somite myotomes by 10.5 days post-coitum.
    • Expression of muscle-specific filamin mRNA preceded myotube formation in vivo.

    Conclusions:

    • Muscle-specific filamin gene expression is initiated early in cardiac and skeletal muscle development, preceding overt myotube differentiation.
    • This study provides the first detailed account of filamin gene expression during early skeletal muscle development stages.
    • The findings contribute to a better understanding of the molecular mechanisms governing myogenesis.