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[The effects of pressure on cultured bovine trabecular meshwork cells]
1Department of Ophthalmology, Union Hospital, Tongji Medical University, Wuhan.
Objective:
To observe the effects of pressure on trabecular meshwork cells.
Methods:
Bovine trabecular meshwork cells were cultured and submitted to different amounts of hydrostatic pressure. Cellular morphology and phagocytic function were observed under inverted phase-contrast microscope, light microscope and electron microscope.
Results:
Compared with the control group, the cells under 2.0 kPa or 2.67 kPa for 48 hours had no remarkable difference in criteria observed. Those under 4.0 kPa for 24 hours showed slight changes in structure and a mild decrease in phagocytic function. The damage appeared more severe if the pressure was higher or lasted longer.
Conclusion:
Trabecular meshwork cells can only bear pressure below a certain level. They may be destroyed structurally or impaired functionally by pressure over this level.
