[The factors affecting apoptosis by in situ terminal deoxynucleotide transferase method]

Apoptosis in biopsies of patients with hepatocirrhosis or hepatocellular carcinoma and in small intestinal mucosa of mice were detected by the method of in situ terminal deoxynucleotide transferase (ISTdT). It was found that the best positive result might be obtained by digesting tissue sections with 25mg x L-1 proteinase K for 15 minutes, then treating sections with moderate degree of microwave for 5 minutes after reaction with terminal deoxynucleotide transferase. The best choice of above conditions were discussed.