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[Patterns of cisplatin induced apoptosis in ovarian cancer cell line COC1]
Objective:
To elucidate the patterns of chemotherapeutic drugs induced apoptosis and its role in cancer chemotherapy.
Methods:
Apoptosis in cultured ovarian cancer cell line COC1 induced by ciplatin was investigated in vitro by applying cytohistochemistry stain, florescence stain and electron microscopy scanning combining with DNA gel electrophoresis and flow cytometry analysis.
Results:
After exposure to cisplatin, COC1 cells manifested typical apoptotic morphological features. Apoptosis persisted throughout 30 hours following addition of cisplatin and augmentated gradually. The rate of apoptosis was enhanced within tested dose range in a dose dependent pattern. At the point of 12 hour cisplatin exposure, the necrosis rate was only 12.4% while the apoptotic rate accounted for 30.8% already.
Conclusions:
Chemotherapeutic drugs induced apoptosis is one of the primary mechanism of anti-cancer chemotherapy.