[Determination of serum copper by atomic absorption spectrometry sensitized with mixed surfactants]

The effects of six kinds of surfactants were studied in the detection of copper in serum by atomic absorption spectrometry. The results demonstrated that the absorption decreased when polyvinyl alcohol was used alone or together with the other one, and the absorption increased when one or two of Tween-20, emulgent-OP, palmityl trimethyl ammonium bromide, arabicgum and sodium laurylsulfate were used. Tween-20 and sodium laurylsulfate used together are most effective. A method of determination of serum copper by atomic absorption spectrometry sensitized with mixture of Tween-20 and sodium laurylsulfate was established. The absorption increased by 50%. The relative standard deviation was 1.35%-2.11%. The recovery was 98.00%-98.13%. The detection limit was 0.0524 mumol/L.