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A spectrophotometric method for assay of tannase using rhodanine
S Sharma1, T K Bhat, R K Dawra
1Department of Chemistry and Biochemistry, College of Basic Sciences, H.P.K.V., Palampur, H.P., India.
Analytical Biochemistry
|February 23, 2000
Abstract:
A method for assay of microbial tannase (tannin acyl hydrolase) based on the formation of chromogen between gallic acid and rhodanine is reported. Unlike the previous protocols, this method is sensitive up to gallic acid concentration of 5 nmol and has a precision of 1.7% (relative standard deviation). The assay is complete in a short time, very convenient, and reproducible.