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Immunogenicity of in vitro folded outer membrane protein PorA of Neisseria meningitidis

C Jansen1, B Kuipers, J van der Biezen

  • 1Department of Molecular Microbiology and Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands.

FEMS Immunology and Medical Microbiology
|February 23, 2000
PubMed

Insights

Researchers developed an endotoxin-free vaccine against serogroup B meningococci. The addition of QuilA adjuvant significantly boosted the immunogenicity of the PorA P1.6 antigen, inducing protective antibodies.

Area of Science:

  • Microbiology
  • Immunology
  • Vaccine Development

Background:

  • PorA P1.6 is a key antigen from Neisseria meningitidis.
  • Previous studies indicated low immunogenicity of purified PorA P1.6 without adjuvants.

Purpose of the Study:

  • To evaluate the immunogenicity of in vitro folded and denatured PorA P1.6 from N. meningitidis strain M990 in mice.
  • To assess the adjuvant effects of QuilA and galE lipooligosaccharide on PorA P1.6 immunogenicity.

Main Methods:

  • In vitro refolding and purification of PorA P1.6 antigen.
  • Immunization of mice with folded/denatured PorA P1.6, with or without QuilA or galE lipooligosaccharide.
  • Analysis of antibody subclasses (IgG2a) and serum bactericidal activity.

Main Results:

  • The immunogenicity of PorA P1.6 was low without adjuvant.
  • QuilA significantly enhanced PorA P1.6 immunogenicity, unlike galE lipooligosaccharide.
  • Immunization with folded PorA P1.6 plus QuilA induced IgG2a antibodies and serum bactericidal activity.

Conclusions:

  • QuilA is an effective adjuvant for enhancing the immunogenicity of PorA P1.6.
  • The developed approach provides a tool for creating endotoxin-free vaccines against serogroup B meningococci.
  • The induction of serum bactericidal activity suggests potential for protective immunity.

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