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Detection of integrins in cataract lens epithelial cells
1International Intraocular Implant Training Centre, Tianjin Medical University, China.
Purpose:
To detect the expression of integrin subunits in lens epithelial cells (LECs) of human cataracts.
Setting:
Research Laboratory, International Intraocular Implant Training Centre, Tianjin Medical University, China.
Methods:
The circular sections of the anterior capsules with attached LECs were obtained during cataract surgery from 100 patients. The LECs were stained with an avidin-biotin-complex immunohistochemical technique using 5 monoclonal antibodies specific for alpha subunits 2, 3, 5 and beta subunits 1, 2.
Results:
All integrin subunits studied were found to varying degrees in specimens. The positive percentages were 70%, 65%, 75%, 70%, and 80%, respectively.
Conclusion:
Integrin subunits were present in LECs of human cataracts. These molecules may serve in the adhesion of LECs to the lens capsule and play a role in cell-posterior capsule interaction after cataract surgery.