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[Application of Apo B 3' DNA polymorphisms in forensic science Practice]
1Department of Physical Evidence Examination, School of Forensic Medicine, Chengdu.
Hua Xi Yi Ke Da Xue Xue Bao = Journal of West China University of Medical Sciences = Huaxi Yike Daxue Xuebao
|June 1, 1997
Summary
Apolipoprotein B (Apo B) 3' VNTR locus analysis accurately genotypes human forensic samples. This DNA profiling method aids in paternity testing and criminal investigations, successfully excluding suspects.
Area of Science:
- Forensic Science
- Genetics
- Molecular Biology
Context:
- DNA analysis is crucial in forensic investigations and paternity testing.
- The Apolipoprotein B (Apo B) 3' VNTR locus is a valuable genetic marker.
- Stability of DNA in various forensic samples is a key consideration.
Purpose:
- To evaluate the utility of Amp-FLP analysis at the Apo B 3' VNTR locus for forensic applications.
- To determine the stability and reliability of Apo B 3' genotypes in different human biological samples.
- To assess the effectiveness of the Apo B 3' locus in paternity testing and criminal case exclusions.
Summary:
- Amp-FLP (Amplification Fragment Length Polymorphism) analysis was performed on the human Apo B 3' VNTR locus using various biological samples including blood stains, saliva, semen, mixed stains, and hair.
- Accurate genotyping was achieved, demonstrating that Apo B 3' locus DNA can be reliably detected in blood stains stored at room temperature for up to 15 weeks and at -20°C for up to two years.
- In paternity testing, the Apo B 3' locus alone or in combination with other markers excluded 6 out of 8 falsely alleged fathers in 34 cases. In rape investigations, 2 out of 3 suspects were excluded.
Impact:
- The Apo B 3' VNTR locus is a robust genetic marker for forensic DNA analysis.
- This method enhances the discriminatory power in paternity testing and forensic casework.
- The stability data supports the use of Apo B 3' locus analysis in real-world forensic scenarios.