[Quantitation of human apolipoprotein B100 by immunoturbidimetric assay]

An immunoturbidimetric assay(ITA) for quantification of human serum apolipoprotein B100 (apoB100) was developed. The assay is sensitive, rapid, specific, easily automatic and thus convenient for routine work. The minimu measurable concentration of apoB100 was 3.1 micrograms in each assay. The standard curve with a working range of 0.34-1.79 g/L was plotted. The between and within assay coefficients of variation were 1.13%-3.48% and 2.91%-4.48% respectively. The recovery was 99.23% +/- 3.57%. The mean serum concentration of apoB100 in 100 normal subjects was 0.821 +/- 0.165 g/L. The correlation coefficient of RID for apoB100 to ITA was 0.8626(P < 0.001).