[A study of immobilized enzyme-assisted semisynthesis of human insulin]

In this paper, a study on preparation of human insulin by immobilized enzyme-assisted semisynthesis is reported for the first time in our country. Porcine insulin with Thr(Bu(t)OB(t) catalyzed by immobilized trypsin has been converted into human insulin via a two-step transpeptidation. With the systematic technique of optimization, the rate of transpeptidation is 66%-70%. PAGE photometry is used to determine the conversion rate of semisynthesis. PAGE and C-terminal analysis of protein are adopted to identify the semisynthesis human insulin.