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Updated: Oct 17, 2026

Milk Collection in the Rat Using Capillary Tubes and Estimation of Milk Fat Content by Creamatocrit
Published on: December 16, 2015
Creamatocrit and the nutrient composition of human milk
C D Wang1, P S Chu, B G Mellen
1Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, TN, USA.
Objective:
We tested the hypothesis that creamatocrit, the length of the cream column separated from milk by centrifugation and expressed as a percentage of the length of the total milk column, is a useful measure of the lipid concentration and the energy content of human milk.
Study Design:
Milk samples from 17 mothers of preterm infants were analyzed prospectively, fresh as well as frozen and thawed, for creamatocrit measurement and nutrient composition.
Results:
Creamatocrit correlated strongly with lipid concentration and energy content of human milk, fresh or frozen and thawed. The energy content can be calculated from the regression equation: Energy (kcal/dl) = 5.99 x creamatocrit(%) + 32.5 for a fresh sample, and energy (kcal/dl) = 6.20 x creamatocrit(%) + 35.1 for a frozen sample.
Conclusion:
Calculations of energy content from the creamatocrit measurement may be useful for an accurate assessment of energy intake in preterm infants fed human milk.
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