Related Experiment Videos
White cell apoptosis in platelet concentrates
F Frabetti1, P L Tazzari, D Musiani
1Immunohematology and Transfusion Service, Policlinico S. Orsola, and the Institute for Histology and General Embryology, University of Bologna, Bologna, Italy.
Transfusion
|February 24, 2000
Summary
Apoptosis of white blood cells (WBCs) occurs in platelet concentrates (PCs) during storage. This process correlates with increased interleukin-8 (IL-8) levels, not platelet activation.
Area of Science:
- Transfusion Medicine
- Cellular Biology
- Immunology
Background:
- Residual white blood cells (WBCs) in platelet concentrates (PCs) can impact transfusion outcomes.
- Understanding WBC apoptosis in PCs is crucial for improving blood product safety and efficacy.
Purpose of the Study:
- To evaluate apoptosis in WBCs within platelet concentrates (PCs).
- To investigate the relationship between WBC apoptosis, platelet activation, and inflammatory cytokine levels in PCs.
Main Methods:
- Analyzed apoptosis in WBCs from 9 PCs (apheresis and pooled buffy coats).
- Assessed platelet activation (CD62P) and cytokine concentrations (IL-1α, IL-1β, IL-6, IL-8, TNF-α) over 4 days of storage.
- Compared irradiated and non-irradiated PC units.
Main Results:
- WBC apoptosis occurred in both irradiated and non-irradiated PCs during storage.
- Platelet activation increased with storage time, particularly in pooled buffy coat PCs.
- Interleukin-8 (IL-8) levels consistently increased with storage time.
Conclusions:
- WBC apoptosis in PCs is time-dependent during storage.
- IL-8 release correlates with WBC apoptosis, suggesting its role as a potential biomarker.
- WBC apoptosis in PCs does not appear directly linked to platelet activation or febrile nonhemolytic transfusion reactions.