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Related Experiment Videos

NAT: perspectives for cellular components.

J A Barbara1

  • 1National Blood Service, North London, Colindale Avenue, London, U.K. john.barbara@nbs.nhs.uk

Biologicals : Journal of the International Association of Biological Standardization
|February 25, 2000
PubMed
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Routine blood screening for Hepatitis C Virus (HCV) RNA using nucleic acid testing (NAT) evolved to ensure safety for both plasma products and blood components. This ensures faster results for labile components like platelets.

Area of Science:

  • Virology
  • Blood Transfusion Safety
  • Public Health

Background:

  • Routine testing for viral genomes in donated blood initially focused on Hepatitis C Virus (HCV) RNA for plasma fractionation.
  • A dual standard for fractionated products and individual blood components was deemed unsustainable.
  • The progression to nucleic acid testing (NAT) for HCV RNA in blood components was implemented in many countries.

Purpose of the Study:

  • To evaluate the necessity and implications of HCV RNA testing for blood component release.
  • To explore the impact of pool sizes on NAT result turnaround time for labile blood components.
  • To assess the feasibility and cost-benefit of expanding NAT to include other viruses like HIV and HBV.

Main Methods:

  • Implementation of routine nucleic acid testing (NAT) for HCV RNA in donated blood.

Related Experiment Videos

  • Analysis of the relationship between sample pool size and the speed of resolving RNA-positive units.
  • Consideration of single sample testing and multiplex assays for broader viral detection.

    • Main Results:

    • HCV RNA testing became standard for blood component release due to its suitability for pooled sample detection.
    • Smaller pool sizes expedite NAT results for labile components but increase costs and laboratory needs.
    • Reduced pool sizes facilitate the potential for testing additional viruses like HIV and HBV.

    Conclusions:

    • HCV RNA NAT is crucial for blood safety, especially for labile components requiring rapid testing.
    • Optimizing pool sizes balances testing efficiency, cost, and the ability to detect multiple viruses.
    • Further assessment of cost-benefit for multiplex assays and alternative HBV screening methods is warranted.