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Related Experiment Videos

Age-dependent increase in C7-1 gene expression in rat frontal cortex.

H Hung1, M J Tsai, H C Wu

  • 1Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan.

Brain Research. Molecular Brain Research
|February 25, 2000
PubMed
Summary

The C7-1 gene, linked to vacuolar H(+)-ATPase (V-ATPase), increases with age in rat brains. Its upregulation may be crucial for maintaining V-ATPase function during aging.

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Area of Science:

  • Neuroscience
  • Molecular Biology
  • Aging Research

Background:

  • Previous studies identified C7-1 mRNA fragment with increased expression in aged rat frontal cortex.
  • The precise function and full sequence of the C7-1 gene were previously unknown.

Purpose of the Study:

  • To clone and sequence the full-length cDNA of the C7-1 gene.
  • To investigate the role of C7-1 in the aging process and its relationship with vacuolar H(+)-ATPase (V-ATPase).

Main Methods:

  • Full-length cDNA cloning and sequencing of the C7-1 gene.
  • Northern blot analysis to assess age-dependent gene expression in different brain regions.
  • Application of C7-1 antisense oligonucleotide in cortical neuronal cultures.

Main Results:

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  • The C7-1 gene encodes a 463-amino-acid protein with 84% sequence identity to a V-ATPase subunit.
  • Age-dependent increase in C7-1 gene expression was observed specifically in the rat frontal cortex.
  • Inhibition of C7-1 expression using antisense oligonucleotides markedly reduced cortical neuronal survival.

Conclusions:

  • C7-1 serves as a molecular marker for the aging process in the brain.
  • Upregulation of C7-1 is potentially important for maintaining normal V-ATPase function during aging.
  • C7-1 plays a role in neuronal survival, particularly in the context of aging.