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Cloning and distribution of the rat parkin mRNA
V D'Agata1, W Zhao, S Cavallaro
1Institute of Bioimaging and Pathophysiology of the Central Nervous System, Italian National Research Council, 95123 Catania, Italy.
Brain Research. Molecular Brain Research
|February 25, 2000
Abstract:
We have isolated by RT-PCR and sequenced a partial cDNA coding for the rat homolog of parkin, a gene mutated in autosomal recessive juvenile parkinsonism. The 1.46 kb rat cDNA clone contains a 1376 bp coding sequence that shares strong similarity with the human parkin cDNA. RT-PCR and in situ hybridization revealed widespread expression of parkin in the rat brain and the periphery. The availability of the rat parkin cDNA and the initial elucidation of its distribution should facilitate further research on the pathophysiological role of parkin in the nervous system.