Related Experiment Video
Updated: Jul 31, 2026

A Flame-Free Method for Sterilizing C. elegans Picks, Spatulas, and Scalpels
Published on: March 4, 2022
Effect of glutaraldehyde-based cold sterilization solutions on light transmission of single-use, plastic light-curing
S K Nelson1, F A Ruggeberg, G A Heuer
1Department of Oral Rehabilitation, School of Dentistry, Medical College of Georgia 30912-1260, USA.
Abstract:
Previous research has demonstrated that autoclaving of conventional curing tips results in the build-up of an opaque scale on the curing tips ends, greatly reducing light intensity output. Cold sterilization of conventional light-curing tips in most glutaraldehyde-based solutions did not damage the tip or decrease light-transmission quality. However, the specific brand of cold sterilant was found to be of importance, as one specific product was shown to decrease light-tip intensity values irreversibly, even after subsequent tip polishing. A decrease in light intensity output from the curing source significantly affects polymer cure and the biological properties of the restorative material.
Related Concept Videos
Cleaning, Sterilization, and Disinfection
Cleaning
The cleaning process usually involves using water with detergents or enzymatic cleaner and removing foreign material from objects and surfaces, including organic material such as body fluids or inorganic material like soil. Cleaning is performed before high-level disinfection and sterilization because foreign materials on the cover of the devices interfere with process...
Methods of Sterilization I: Physical Methods
Steam sterilization uses non-toxic, low-cost moist heat in the form of saturated steam under pressure, which is fast, microbicidal, and sporicidal, and quickly warms and penetrates fabrics. Autoclaves, or steam sterilizers, expose each item to direct steam contact for a predetermined time at the necessary...
Methods of Sterilization II: Chemical Methods
Using chemical sterilization rather than heat to clean out equipment is recommended. It eradicates and removes all bacteria,...
Physical Methods for Controlling Microbial Growth: Radiation and Filtration

