Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Signal transduction pathways activated by CpG-DNA.

H Häcker1

  • 1Institut für Med. Mikrobiologie, Immunologie and Hygiene, Technische Universität München, Germany. hans.haecker@lrz.tum.de

Current Topics in Microbiology and Immunology
|February 26, 2000
PubMed
Summary

CpG-DNA acts as an adjuvant by activating NF-kappa B and stress-kinase pathways, likely through a specific receptor. Further research is needed to fully understand its mechanism and physiological role.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nocebo effect of informed consent: circulatory collapse before elective caesarean section.

International journal of obstetric anesthesia·2016
Same author

Survival and differentiation defects contribute to neutropenia in glucose-6-phosphatase-β (G6PC3) deficiency in a model of mouse neutrophil granulocyte differentiation.

Cell death and differentiation·2013
Same author

Molecular analysis of neutrophil spontaneous apoptosis reveals a strong role for the pro-apoptotic BH3-only protein Noxa.

Cell death and differentiation·2011
Same author

Differential regulation by estrogen of C-fos in hamster-kidney and estrogen-induced kidney tumor-cells - receptor mediation vs metabolic-activation.

International journal of oncology·2011
Same author

Changes in liver glycogen and lipid metabolism during transient graft-versus-host (GvH) and graft-versus-leukemia (GvL) reactivity.

International journal of oncology·2011
Same author

Focal hepatic glycogenosis.

International journal of oncology·2011

Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • CpG-DNA is recognized for its adjuvant properties, but its precise molecular mechanisms remain unclear.
  • CpG-DNA utilizes known signaling pathways like NF-kappa B and stress-kinase pathways.
  • These pathways lead to the induction of transcriptional activity for NF-kappa B and AP-1.

Purpose of the Study:

  • To investigate the molecular mechanism of CpG-DNA as an adjuvant.
  • To determine how CpG-DNA signaling integrates into existing cellular pathways.
  • To explore the potential existence of a CpG-DNA-sequence-specific receptor.

Main Methods:

  • Analysis of NF-kappa B and stress-kinase pathways activation by CpG-DNA.
  • Investigation of cell-type specificity in CpG-DNA response.
  • Use of competing non-CpG oligonucleotides (ODNs) and endosomal acidification inhibitors.
  • Distinguishing CpG-dependent from CpG-independent activities.

Main Results:

  • CpG-DNA signaling enters known pathways, similar to stimuli like LPS, IL-1, and TNF.
  • Evidence suggests a CpG-DNA-sequence-specific receptor due to motif dependency and cell-type specificity.
  • CpG ODNs are endocytosed via a receptor-independent mechanism, followed by a pH-dependent signaling step.
  • CpG-independent activities of DNA, particularly with poly-G stretches, were observed.

Conclusions:

  • CpG-DNA signaling is initiated through known pathways, with evidence supporting a specific receptor.
  • A model involving endosomal uptake and pH-dependent signaling is proposed.
  • Distinguishing CpG-dependent from CpG-independent DNA activities is crucial.
  • Further understanding of CpG-receptor interactions is essential for elucidating the physiological role of CpG-DNA.

Related Experiment Videos