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Shufflons: multiple inversion systems and integrons.

¹Department of Biology, Tokyo Metropolitan University, Japan. komano-teruya@c.metro-u.ac.jp

Annual Review of Genetics

|February 26, 2000

Summary

The R64 shufflon uses site-specific recombination to generate biological diversity in prokaryotes. This system acts as a biological switch, influencing pilus protein components and recipient specificity in plasmid R64 liquid matings.

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Area of Science:

Microbiology
Molecular Biology
Genetics

Background:

Site-specific recombination is a key mechanism for generating biological diversity in prokaryotes.
The plasmid R64 shufflon comprises seven recombination sites flanking four DNA segments, enabling complex genetic rearrangements.

Purpose of the Study:

To elucidate the function of the R64 shufflon in prokaryotic biological diversity.
To explain the role of the shufflon as a biological switch in regulating gene expression and protein components.
To describe the shufflon's impact on recipient specificity during plasmid R64 liquid matings.

Main Methods:

Analysis of site-specific recombination events mediated by the rci gene product.
Investigation of DNA segment inversions within the shufflon.
Functional studies on the shufflon's role in selecting C-terminal segments of PilV proteins.

Main Results:

Site-specific recombination within the shufflon leads to the inversion of four DNA segments, either individually or collectively.
The shufflon functions as a biological switch, determining the selection of one of seven C-terminal segments of PilV proteins.
The shufflon dictates recipient specificity in liquid matings involving plasmid R64.

Conclusions:

The R64 shufflon is a sophisticated system for generating biological diversity through DNA inversion.
This mechanism allows for the regulation of protein components, such as PilV, and influences bacterial conjugation specificity.
The review also touches upon other multiple inversion and insertion systems like integrons.

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