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Apolipoprotein E polymorphism and plasma lipid levels in Native Mongolian sheep.

K Tsunoda1, K Nozawa, Y Maeda

  • 1Showa University School of Medicine, Tokyo, Japan.

Biochemical Genetics
|February 26, 2000
PubMed
Summary
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Researchers identified 28 apolipoprotein E (apoE) phenotypes in Mongolian Khalkhas sheep, discovering four new apoE variants. Specific apoE genotypes correlated with higher plasma cholesterol levels, offering insights into sheep lipid metabolism.

Area of Science:

  • Genetics
  • Biochemistry
  • Animal Science

Background:

  • Apolipoprotein E (apoE) plays a crucial role in lipid metabolism.
  • Understanding apoE polymorphism in livestock is important for breeding and health management.
  • Previous studies have characterized apoE in various sheep breeds, but data on Mongolian Khalkhas sheep is limited.

Purpose of the Study:

  • To determine the apolipoprotein E (apoE) phenotypes in native Mongolian Khalkhas sheep.
  • To identify and characterize new apoE variants and their genetic control.
  • To investigate the association between different apoE phenotypes and plasma lipid levels.

Main Methods:

  • Polyacrylamide gel isoelectric focusing-immunoblotting technique for apoE phenotyping.
  • Enzymatic assays for quantifying plasma lipid levels (total cholesterol, LDL cholesterol).

Related Experiment Videos

  • Population genetics analysis to determine allele frequencies and inheritance patterns.

    • Main Results:

    • Twenty-eight apoE phenotypes were identified in 199 Khalkhas sheep.
    • Four novel apoE variants (E8, E9, E10, E11) were discovered, controlled by four codominant alleles (APOE8-11).
    • Allele frequencies for the new variants ranged from 0.005 to 0.0126.
    • Khalkhas sheep apoE allele frequencies differed significantly from other Asian sheep breeds.
    • Sheep with type 1/1 and/or 2/7 apoE phenotypes exhibited significantly higher plasma total and LDL cholesterol compared to type 7/7.

    Conclusions:

    • The Mongolian Khalkhas sheep population exhibits significant apoE genetic diversity, including novel variants.
    • Apolipoprotein E genotype is a significant determinant of plasma lipid profiles in Khalkhas sheep.
    • Findings contribute to understanding apoE genetics in Asian sheep and have implications for lipid metabolism research.