Related Experiment Video
Updated: Oct 17, 2026

A Sensitive Visual Method for the Detection of Hydrogen Sulfide Producing Bacteria
Published on: June 27, 2022
Improved plating media for the detection of Salmonella species with typical and atypical hydrogen sulfide production
E T Mallinson1, R G Miller, C E de Rezende
1Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland Campus, College Park MD 20742-3711, USA.
Abstract:
The Salmonella detection ability of 2 surfactant-supplemental media, xylose-lysine-tergitol (Nia-proof) 4 (XLT4) and Miller-Mallinson (MM) agar, was compared against that of several commonly used plating media. XLT4 and MM appeared to be the most efficient in detecting Salmonella in meat products and food animal environments. MM was superior to XLT4 in detecting those increasingly more prevalent strains of Salmonella possessing weak to ultraweak H2S production characteristics.

