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Related Experiment Videos

The skin produces urocortin.

A Slominski1, B Roloff, J Curry

  • 1Department of Pathology, Loyola University Medical Center, Maywood, Illinois 60153, USA. aslomin@wpo.it.luc.edu

The Journal of Clinical Endocrinology and Metabolism
|February 26, 2000
PubMed
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Human skin produces urocortin, a peptide related to corticotropin-releasing hormone (CRH). This study confirms functional expression of the urocortin gene and peptide production in skin cells and melanomas.

Area of Science:

  • Dermatology
  • Endocrinology
  • Molecular Biology

Background:

  • The skin is known to produce pro-opiomelanocortin (POMC) peptides.
  • Corticotropin-releasing hormone (CRH) is the endogenous stimulant for POMC.
  • Urocortin is a peptide related to CRH, and its role in skin was previously unclear.

Purpose of the Study:

  • To investigate the local production of urocortin in human and mouse skin.
  • To determine if skin cells, including keratinocytes and melanoma cells, express the urocortin gene and produce the urocortin peptide.

Main Methods:

  • Radioimmunoassay (RIA) to detect urocortin immunoreactivity.
  • Reverse-phase high-performance liquid chromatography (RP-HPLC) for peptide separation and confirmation.
  • Liquid chromatography-mass spectrometry (LC-MS) for identification.

Related Experiment Videos

  • Immunohistochemistry for peptide localization.
  • Reverse transcription-polymerase chain reaction (RT-PCR) and gene sequencing to analyze urocortin gene expression.

    • Main Results:

    • Urocortin immunoreactivity was detected in human and mouse skin extracts, cultured keratinocytes, and melanoma cells.
    • RP-HPLC and LC-MS confirmed the presence and identity of urocortin in skin.
    • Immunolocalization revealed urocortin in keratinocytes, glands, dermal muscle, nevocytes, and various skin cancer cells.
    • RT-PCR and sequencing confirmed the expression of the urocortin gene in all tested skin-derived samples.

    Conclusions:

    • Human and mouse skin exhibit functional expression of the urocortin gene.
    • Skin cells, including keratinocytes and melanoma cells, actively produce the urocortin peptide.
    • These findings highlight a novel role for urocortin in skin physiology and pathology.