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Synthesis of (E)-9-(1-pyrenyl)-4-hydroxynon-2-enal, a fluorescent probe of the (E)-4-hydroxynon-2-enal with retained
P Allevi1, M Anastasia, F Cajone
1Departimento di Chimica e Biochimica Medica, Universita degli Studi diMilano, Italy. pietro.allevi@ unimi.it
Chemistry and Physics of Lipids
|February 26, 2000
Abstract:
(E)-9-(1-pyrenyl)-4-hydroxynon-2-enal (FHNE), a fluorescent probe of (E)-4-hydroxynon-2-enal (HNE) is synthesised in seven steps and in 35% overall yield, starting from commercially available 1-pyrencarboxyaldehyde. When incubated with cultured HeLa cells this fluorescent probe penetrates cells and particularly concentrates in the region surrounding the nucleus. As the parent compound, HNE it is able to induce the activation of heat shock factor (HSF) and is able to induce the binding of HSF to heat shock element (HSE).