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Published on: May 19, 2012
Microbial glucosylation of thaxtomin A, a partial detoxification
R R King1, C H Lawrence, L A Calhoun
1Department of Chemistry, University of New Brunswick, Fredericton, New Brunswick, Canada E3B 6E2.
Bacillus mycoides biotransformed thaxtomin A and B, the potato common scab toxins, into less harmful beta-glucosides. This discovery offers potential for managing this significant plant disease.
Area of Science:
- Plant Pathology
- Microbial Biotechnology
- Biochemistry
Background:
- Common scab, a potato disease, is caused by phytotoxins thaxtomin A and B.
- Understanding the degradation pathways of these toxins is crucial for disease management.
Purpose of the Study:
- To investigate the biotransformation of thaxtomin A and B by Bacillus mycoides.
- To assess the phytotoxicity of the resulting biotransformation products.
Main Methods:
- Incubation of thaxtomin A and B with Bacillus mycoides cultures in oatmeal broth.
- Biotransformation of toxins into C-14 linked beta-glucosides.
- Assay of phytotoxicity on aseptically produced potato minitubers.
Main Results:
- Thaxtomin A and B were successfully biotransformed into beta-glucosides (3 and 4).
- The resulting beta-glucosides exhibited significantly reduced phytotoxicity compared to the parent thaxtomin compounds.
Conclusions:
- Bacillus mycoides can detoxify thaxtomin A and B through beta-glucosidation.
- This microbial biotransformation pathway holds potential for developing strategies to control potato common scab.
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