Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Collagen fibrillogenesis: intermediate aggregates and suprafibrillar order.

R L Trelstad, K Hayashi, J Gross

    Proceedings of the National Academy of Sciences of the United States of America
    |November 1, 1976
    PubMed
    Summary

    Collagen polymerization in vitro reveals distinct aggregation stages during fibril formation. Electron microscopy shows linear and lateral growth via subfibril addition and entwining, forming ordered structures.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Mini-asterixis in hepatic encephalopathy induced by pathologic thalamo-motor-cortical coupling.

    Neurology·2003
    Same author

    Properties of MEG tomographic maps obtained with spatial filtering.

    NeuroImage·2003
    Same author

    Latin-American trial of orlistat for weight loss and improvement in glycaemic profile in obese diabetic patients.

    Diabetes, obesity & metabolism·2003
    Same author

    [The cadherin hypothesis of schizophrenia].

    Fortschritte der Neurologie-Psychiatrie·2003
    Same author

    Nicotinamide prevents the long-term effects of perinatal asphyxia on basal ganglia monoamine systems in the rat.

    Experimental brain research·2003
    Same author

    Differential response of immature and mature neurons to hypoxia in rat mesencephalic cultures.

    Developmental neuroscience·2002

    Area of Science:

    • Biochemistry
    • Biophysics
    • Materials Science

    Background:

    • Collagen is a crucial structural protein in connective tissues.
    • Understanding collagen polymerization is vital for tissue engineering and biomaterials development.
    • In vitro studies are essential for dissecting the early stages of fibrillogenesis.

    Purpose of the Study:

    • To investigate the early stages of collagen polymerization in vitro.
    • To identify and characterize distinct morphological stages of fibril assembly.
    • To elucidate the mechanisms of linear and lateral fibril growth.

    Main Methods:

    • Electron microscopy was used to observe collagen aggregation.
    • In vitro polymerization experiments were conducted.
    • Time-resolved morphological analysis of fibril assembly was performed.

    Related Experiment Videos

    Main Results:

    • Morphologically distinct stages of collagen aggregation were identified.
    • Linear fibril growth occurs through tandem addition of aggregates and subfibrils.
    • Lateral fibril growth involves the entwining of subfibrils.
    • Suprafibrillar order, including parallel, spiral, and orthogonal arrangements, was observed.

    Conclusions:

    • The study identified sequential stages in collagen fibril formation.
    • A model for collagen fibrillogenesis involving subfibril addition and entwining is proposed.
    • The observed suprafibrillar order suggests complex self-assembly mechanisms in collagen polymerization.