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Determination of histamine in tomatoes by capillary electrophoresis
E Bolygo1, P A Cooper, K M Jessop
1Zeneca Agrochemicals, Jealotts Hill Research Station, Bracknell, Berkshire, UK.
Journal of AOAC International
|February 29, 2000
Abstract:
An improved capillary electrophoresis assay for histamine in crude extracts was developed and used to determine histamine levels in a selection of tomato fruits and pastes. Performance in terms of reproducibility and sensitivity was optimized by use of a high sensitivity detector flow cell, sodium hydroxide rinses, and a voltage gradient. The method was linear down to 0.2 microgram/mL (signal-to-noise ratio = 4:1), which was below the endogenous level in all samples.