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Superstructure of linear duplex DNA.

H J Vollenweider, T Koller, J Parello

    Proceedings of the National Academy of Sciences of the United States of America
    |November 1, 1976
    PubMed
    Summary
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    Linear duplex DNA (T7) forms superhelical structures similar to closed circular DNA (PM2) at high ionic strength. This DNA superstructure is disrupted by heat or ethidium bromide.

    Area of Science:

    • Molecular Biology
    • Biophysics

    Background:

    • Covalently closed circular DNA (cccDNA) exhibits superhelical structures.
    • Linear duplex DNA's structural behavior under varying conditions is less understood.

    Purpose of the Study:

    • To compare the superstructure of PM2 cccDNA with T7 linear duplex DNA.
    • To investigate the influence of ionic strength and surfactant concentration on DNA structure.

    Main Methods:

    • Electron microscopy was used to visualize DNA superstructures.
    • Analytical ultracentrifugation assessed DNA sedimentation behavior.

    Main Results:

    • Increasing ionic strength induced linear T7 DNA to form structures resembling PM2 cccDNA's superhelix.
    • DNA superstructure was destabilized under premelting conditions.

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  • Ethidium bromide disrupted the observed DNA superstructures.

    • Conclusions:

    • Linear duplex DNA can spontaneously adopt superhelical conformations.
    • Ionic strength is a critical factor in DNA structural transitions.
    • DNA superstructures are sensitive to thermal and chemical denaturation.