Allelic heterogeneity within allozymes separated by electrophoresis in Drosophila pseudoobscura

Electrophoretic separation of esterase-5 allozymes of Drosophila pseudoobscura in gels of different acrylamide concentrations and in different buffer systems has revealed the existence of six different alleles within the most frequent class, esterase-5(1.00), and three alleles in the other most frequent class, esterase-5(1.07). Two of the alleles affect subunit binding. Esterase-5 allozyme classes based on electrophoresis in 5% acrylamide gels are therefore highly heterogeneous.