Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Heat mutagenesis in bacteriophage T4: the transversion pathway.

P M Bingham, R H Baltz, L S Ripley

    Proceedings of the National Academy of Sciences of the United States of America
    |November 1, 1976
    PubMed
    Summary

    Heat causes genetic mutations called transversions at guanine bases in bacteriophage T4 DNA. A proposed model suggests a structural change in guanine leads to these G-C to C-G mutations.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Complex regional pain syndrome in pediatric patients with severe factor VIII deficiency.

    Journal of pediatric hematology/oncology·2002
    Same author

    Genetic methods and strategies for secondary metabolite yield improvement in actinomycetes.

    Antonie van Leeuwenhoek·2002
    Same author

    Genes for the biosynthesis of spinosyns: applications for yield improvement in Saccharopolyspora spinosa.

    Journal of industrial microbiology & biotechnology·2002
    Same author

    Genome of the bacterium Streptococcus pneumoniae strain R6.

    Journal of bacteriology·2001
    Same author

    Genetic fidelity under harsh conditions: analysis of spontaneous mutation in the thermoacidophilic archaeon Sulfolobus acidocaldarius.

    Proceedings of the National Academy of Sciences of the United States of America·2001
    Same author

    Conditional coupling of leading-strand and lagging-strand DNA synthesis at bacteriophage T4 replication forks.

    The Journal of biological chemistry·2001

    Area of Science:

    • Molecular Biology
    • Genetics
    • Biochemistry

    Background:

    • Heat is a known mutagenic agent.
    • DNA base pair changes can lead to mutations.
    • Bacteriophage T4 is a model organism for studying DNA replication and repair.

    Purpose of the Study:

    • To investigate the specific types of DNA mutations induced by heat in bacteriophage T4.
    • To elucidate the molecular mechanism underlying heat-induced transversions at G-C base pairs.
    • To propose a model for heat-induced guanine modification and subsequent mispairing.

    Main Methods:

    • Analysis of mutation spectra in bacteriophage T4 exposed to heat.
    • Biochemical assays to identify heat-induced DNA adducts.
    • Modeling of DNA base pairing interactions.

    Related Experiment Videos

    Main Results:

    • Heat induces both transitions and transversions at guanine-cytosine (G-C) base pairs.
    • The primary target for transversions is guanine, which is modified into a product misread as a pyrimidine during replication.
    • A proposed model involves the migration of the deoxyguanosine glycosidic bond, leading to G-C to C-G transversions.

    Conclusions:

    • Heat-induced DNA damage in bacteriophage T4 involves specific modifications to guanine.
    • The proposed N9 to N2 glycosidic bond migration model explains the observed G-C to C-G transversions.
    • Understanding these mechanisms is crucial for comprehending heat mutagenesis.