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Related Experiment Videos

Nonenzymatic elimination of ascorbic acid in clinical samples.

Y Kayamori1, Y Katayama, T Urata

  • 1Department of Clinical Chemistry, National Cardiovascular Center, Osaka, Japan. ykayamor@hsp.ncvc.go.jp

Clinical Biochemistry
|February 29, 2000
PubMed
Summary

Stable radicals, termed ascorbic acid quenchers (AAQs), effectively eliminate ascorbic acid interference in uric acid assays. AAQ-2 demonstrated superior quenching ability compared to traditional ascorbate oxidase, proving suitable for clinical diagnostics.

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Area of Science:

  • Biochemistry
  • Clinical Chemistry
  • Analytical Chemistry

Background:

  • Ascorbic acid interferes with oxidative chromogenic reagents in peroxidase/hydrogen peroxide reactions.
  • Ascorbate oxidase is a common but costly and unstable solution for ascorbic acid interference.
  • Novel, stable radicals (ascorbic acid quenchers, AAQs) offer a nonenzymatic alternative.

Purpose of the Study:

  • To evaluate stable radicals (AAQs) as nonenzymatic ascorbic acid quenchers.
  • To assess AAQ efficacy in uricase-based uric acid determination in serum and urine.
  • To compare AAQ performance against traditional ascorbate oxidase.

Main Methods:

  • Utilized a commercially available uric acid detection kit.
  • Employed the TBA-80FR.NEO biochemical analyzer for assays.

Related Experiment Videos

  • Tested four stable radicals for ascorbic acid quenching activity.

    • Main Results:

    • 4-Hydroxy-2,2,6,6-tetramethyl-1-piperidinyloxy free radical (AAQ-2) was the most effective quencher.
    • AAQ-2 did not interfere with the uric acid assay.
    • 2 mmol/L AAQ-2 exhibited quenching ability superior to or equal to 2 U/ml ascorbate oxidase.

    Conclusions:

    • AAQ-2 is a suitable and effective nonenzymatic quencher for ascorbic acid in clinical samples.
    • AAQ-2 offers a stable and potentially more cost-effective alternative to ascorbate oxidase.
    • This method improves the reliability of uric acid determination in biological fluids.