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Nitric oxide generation by soybean embryonic axes. Possible effect on mitochondrial function.

A Caro1, S Puntarulo

  • 1Physical Chemistry-PRALIB, School of Pharmacy and Biochemistry, University of Buenos Aires, Argentina.

Free Radical Research
|February 29, 2000
PubMed
Summary
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Nitric oxide (NO) influences soybean germination by affecting mitochondrial oxygen consumption. This study reveals NO

Area of Science:

  • Plant Physiology
  • Biochemistry
  • Molecular Biology

Background:

  • Nitric oxide (NO) is a signaling molecule involved in various plant processes.
  • Mitochondrial respiration is crucial for energy production during seed germination.

Purpose of the Study:

  • To investigate nitric oxide generation during soybean germination.
  • To determine the effect of NO on mitochondrial enzyme activity and oxygen consumption in soybean embryonic axes.

Main Methods:

  • Electron Paramagnetic Resonance (EPR) spectroscopy for NO detection.
  • Measurement of nitrate reductase and NADPH-diaphorase activities.
  • Assessment of oxygen consumption in isolated mitochondria.

Main Results:

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  • NO levels and related enzyme activities peaked at 15-24 hours of imbibition.
  • Nitric oxide inhibited mitochondrial oxygen consumption in a dose-dependent manner.
  • Cytochrome oxidase was more sensitive to NO than the alternative oxidase pathway.
  • NO treatment increased hydrogen peroxide production in mitochondria.

Conclusions:

  • Nitric oxide modulates oxygen consumption in soybean embryonic axes during germination.
  • NO may influence the pro-oxidant/antioxidant balance and cellular energy yield.
  • NO plays a potential role in regulating soybean germination.