Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Antibody modeling: implications for engineering and design.

V Morea1, A M Lesk, A Tramontano

  • 1IRBM "P. Angeletti,", Via Pontina Km. 30.600, Pomezia, 00040, Italy.

Methods (San Diego, Calif.)
|March 1, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Snail repressor recruits EZH2 to specific genomic sites through the enrollment of the lncRNA HOTAIR in epithelial-to-mesenchymal transition.

Oncogene·2016
Same author

A cross-sectional nationwide survey on esophageal atresia and tracheoesophageal fistula.

Journal of pediatric surgery·2015
Same author

SAP97-mediated ADAM10 trafficking from Golgi outposts depends on PKC phosphorylation.

Cell death & disease·2014
Same author

Mapping the hydropathy of amino acids based on their local solvation structure.

The journal of physical chemistry. B·2014
Same author

Detection of blood pressure increments in active elderly individuals: the role of ambulatory blood pressure monitoring.

Nutrition, metabolism, and cardiovascular diseases : NMCD·2014
Same author

Covalent binding antibodies suppress advanced glycation: on the innate tier of adaptive immunity.

Acta naturae·2012
Same journal

An accessible, absorbance-based plate reader assay to assess cumulative exposure of blood plasma & serum to thawed conditions.

Methods (San Diego, Calif.)·2026
Same journal

EC-isHCR: A rapid method for in situ hybridization chain reaction in diverse animal samples.

Methods (San Diego, Calif.)·2026
Same journal

Single-Molecule methods to investigate mechanisms of transcription by RNA polymerase of Mycobacterium tuberculosis.

Methods (San Diego, Calif.)·2026
Same journal

Detection and sequencing of Usutu virus during mosquito surveillance: Use of multiple assays and techniques for identification at low levels.

Methods (San Diego, Calif.)·2026
Same journal

Experimental validation of an AI-driven digital healthcare platform for oral health behavior and plaque assessment among vietnamese children.

Methods (San Diego, Calif.)·2026
Same journal

Zeta potential: An efficient and cost-effective alternative for investigating cell-surface interactions.

Methods (San Diego, Calif.)·2026
See all related articles

Accurate antibody modeling procedures predict antibody structures using sequence-to-structure rules. This knowledge enables engineering antibody-like molecules for therapeutic and research applications, including antibody humanization.

Area of Science:

  • Immunology
  • Structural Biology
  • Bioengineering

Background:

  • Antibody structure-sequence relationships are crucial for understanding antibody function.
  • Knowledge-based approaches have enabled the engineering of novel antibody-like molecules with tailored properties.
  • These engineered molecules have significant therapeutic and research potential.

Purpose of the Study:

  • To describe a knowledge-based procedure for predicting antibody variable domains.
  • To evaluate the accuracy and limitations of this prediction method.
  • To illustrate rational antibody design using antibody humanization as an example.

Main Methods:

  • Utilizing sequence-to-structure rules for antibody modeling.
  • Employing the canonical structures method for antigen-binding site prediction.

Related Experiment Videos

  • Analyzing existing antibody structures to inform modeling procedures.

    • Main Results:

    • Development of accurate knowledge-based procedures for antibody modeling.
    • Successful engineering of antibody-like molecules with enhanced stability and specificity.
    • Demonstration of antibody humanization as a key application in reducing immunogenicity.

    Conclusions:

    • Understanding antibody sequence-structure relationships is key to accurate modeling.
    • Knowledge-based antibody modeling facilitates the rational design of therapeutic agents.
    • Antibody engineering, including humanization, offers broad applications in medicine and research.