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Determination of phenobarbital in plasma by micellar liquid chromatography
Y Martín-Biosca1, S Sagrado, R M Villanueva-Camãnas
1Departamento de Química Analítica, Facultad de Farmacia, Universitat de València, C/ Vicente A. Estellés s/n, E-46100 Burjassot, València, Spain.
Abstract:
A new liquid chromatographic procedure for the determination of phenobarbital in plasma samples is described. The proposed system uses a Spherisorb octadecyl-silane ODS-2 C(18) analytical column, a guard column of similar characteristics, and a 0.03 M CTAB-3% 1-propanol at pH 7 mobile phase. The UV detector was set at 250 nm. Butabarbital was used as internal standard. Sample preparation only required the addition to the plasma samples of a 0.1 M SDS solution at pH 3 and centrifugation before injection into the chromatographic system. The limit of detection was 0.83 microg/mL of phenobarbital in plasma samples. The coefficients of variation were lower than 7. 5%.
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