Related Experiment Videos
Growth factor mRNA and protein in preserved human amniotic membrane
N J Koizumi1, T J Inatomi, C J Sotozono
1Department of Ophthalmology, Kyoto Prefectural University of Medicine, Japan. nkoizumi@eye.ophth.kpu-m.ac.jp
Current Eye Research
|March 1, 2000
Summary
Preserved human amniotic membrane (AM) contains growth factors like EGF and KGF that support epithelialization. AM with epithelium shows higher levels of these key growth factors, aiding ocular surface wound healing.
Area of Science:
- Ophthalmology
- Regenerative Medicine
- Molecular Biology
Background:
- Human amniotic membrane (AM) is utilized in ocular surface reconstruction.
- Understanding the molecular composition of preserved AM is crucial for its therapeutic efficacy.
Purpose of the Study:
- To investigate the expression of growth factor mRNA and protein levels in preserved human amniotic membrane (AM).
Main Methods:
- Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect mRNA for eight growth factors and two receptors.
- Enzyme-linked immunosorbent assays (ELISAs) quantified protein concentrations of seven growth factors in AM and corneas.
Main Results:
- Preserved AM expressed mRNA for multiple growth factors (EGF, TGF-alpha, KGF, HGF, bFGF, TGF-beta1, -beta2, -beta3) and receptors (KGFR, HGFR).
- AM contained detectable levels of EGF, TGF-alpha, KGF, HGF, bFGF, TGF-beta1, and -beta2 proteins.
- AM with amniotic epithelium exhibited significantly higher protein levels of EGF, KGF, HGF, and bFGF compared to AM without epithelium.
Conclusions:
- Preserved human AM contains growth factors beneficial for epithelialization after transplantation.
- The amniotic epithelium appears to be the source of key growth factors like EGF, KGF, and HGF.
- These growth factors, particularly EGF, KGF, and HGF, likely play a significant role in ocular surface wound healing.