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Rat Burn Model to Study Full-Thickness Cutaneous Thermal Burn and Infection
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Bacteriological analysis of burn sepsis
B S Nagoba1, S R Deshmukh, B J Wadher
1Microbiology Dept, M.I.M.S.R. Medical College, Latur.
Abstract:
A total of 114 opportunistic bacteria were isolated from 65 swabs from burn sepsis. P. aeruginosa (53.8%) was the most common agent followed by S. aureus (38.4%), Klebsiella Spp. (27.6%), Proteus (18.4%), E. coil (10.7%) and others. The infection was monobacterial in 25 cases (38.4%) and polybacterial in 49 cases (61.5%). P. aeruginosa was predominated in both monobacterial and polybacterial infections. Ciprofloxacin (42.9%) was found to be the most effective antibacterial agent. Results indicate that resistance in burn isolates is higher and increasing day by day.
Insights
Burn sepsis infections commonly involve opportunistic bacteria like Pseudomonas aeruginosa and Staphylococcus aureus. Ciprofloxacin showed effectiveness, but increasing bacterial resistance is a significant concern.
Area of Science:
- Medical Microbiology
- Infectious Diseases
- Antimicrobial Resistance
Background:
- Burn wound infections are a significant cause of morbidity and mortality.
- Opportunistic bacteria frequently cause burn sepsis, leading to complications.
- Understanding the prevalent pathogens and their antimicrobial susceptibility is crucial for effective treatment.
Purpose of the Study:
- To identify the spectrum of opportunistic bacteria isolated from burn sepsis cases.
- To determine the prevalence of monobacterial versus polybacterial infections.
- To evaluate the in vitro efficacy of common antibacterial agents against these isolates.
Main Methods:
- Bacterial isolation and identification from 65 burn sepsis patient swabs.
- Antimicrobial susceptibility testing of isolated bacterial strains.
- Analysis of infection types (monobacterial vs. polybacterial) and predominant pathogens.
Main Results:
- 114 opportunistic bacteria were isolated, with Pseudomonas aeruginosa (53.8%) and Staphylococcus aureus (38.4%) being the most common.
- Polybacterial infections (61.5%) were more frequent than monobacterial infections (38.4%).
- Ciprofloxacin (42.9%) demonstrated the highest efficacy, yet overall resistance rates were notably high and increasing.
Conclusions:
- Pseudomonas aeruginosa is the predominant pathogen in burn sepsis, often in polymicrobial settings.
- Increasing antimicrobial resistance poses a significant challenge in managing burn sepsis.
- Continuous monitoring of bacterial susceptibility patterns is essential for guiding empirical therapy.
